Gene expression profiles of human adipose-derived mesenchymal stem cells dynamically seeded on clinically available processed nerve allografts and collagen nerve guides

It was hypothesized that mesenchymal stem cells (MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine gene expression MSCs influenced by Avance® Nerve Grafts or NeuraGen® Guides. Human adipose-derived were cultured and dynamically 30 Guides for 12 hours. At six time points after seeding, quantitative polymerase chain reaction analyses performed five samples per group. Neurotrophic [nerve growth factor (NGF), glial cell line-derived neurotrophic (GDNF), pleiotrophin (PTN), associated protein 43 (GAP43) brain-derived (BDNF)], myelination [peripheral myelin 22 (PMP22) zero (MPZ)], angiogenic [platelet endothelial adhesion molecule 1 (PECAM1/CD31) vascular alpha (VEGFA)], extracellular matrix (ECM) [collagen type I (COL1A1), collagen III (COL3A1), Fibulin (FBLN1) laminin subunit beta 2 (LAMB2)] surface marker cluster differentiation 96 (CD96) quantified. Unseeded evaluate baseline expression, unseeded provided MSCs. The interaction with led a short-term upregulation (NGF, GDNF BDNF), (PMP22 MPZ) genes (CD31 VEGFA) long-term BDNF, VEGFA COL1A1. between Guide short term BDNF) MPZ), VEGFA), ECM (COL1A1) (GDNF (COL1A1, COL3A1, FBLN1) genes. Analysis demonstrated expressed significantly higher levels (VEGFA) (COL3A1, in long period compared Grafts. Overall, human both substitutes resulted significant numerous important regeneration over time. vitro more pronounced, particularly (> 14 days seeding). These results suggest MSC-seeding has potential be applied clinical setting, which needs confirmed future vivo research.